Family Background And History That May Affect Planning And...

FAMILY UPDATE Family Background Describe relevant family background and history that may affect planning and service provision for this family. Ms. Goodall was adoptive as a child. She reports that she knows some of her family on her biological side of the family, but not communicates with them on regular bases. However, Ms. Goodall seems to not get along with her adoptive parents. She still resides in the home even though there is an order protection against her adoptive father, Mr. Goodall. Ms. Goodall is currently engaged as a home health aide and also receives some financial support from her boyfriend, Mr. Ruby. Case Update Summarize the key family events, services, and casework activities since the last Family Assessment and Service Plan. Include legal activities, if any. Reason for Referral/ Presenting Problem/ Mandated Services Ms. Goodall walked into HDWC on 02/11/2015 for assistance. She was referred by ACS Community Representative. She requested for assistance to address her concerns of childcare for Myan, housing, early intervention, and mental health services to address her bi-polar and depression to address problems within her family. Ms. Goodall admits to discontinuing mental health services, including her prior prescribed medication. In addition, engaging in substance abuse while pregnant with Myan. Living Environment Ms. Goodall living situation is not stable. She at first did not have any place to stay for herself and her daughter, Myan.Show MoreRelatedWhy Is Renting Breathable For People?1247 Words   |  5 Pagesenable affordability; and those regulations are to be monitored by a trusted government agency. 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Founded in October 1994, the ADA Foundation was created to substantially accelerate the Association s ability to raise major gifts to directlyRead MoreReal Estate Properties Of Public Records1591 Words   |  7 Pagesplaces they are familiar with, subsequently they are aware of issues that may affect the property s value. Appraisers usually value one property at a time, and they can often specialize in certain types of real estate. (Appraisal Careers) Commercial appraisers specialize in working with income producing property like stores, hotels, and office buildings. Residential appraisers focus on appraising personal property like single family homes and condominiums, appraising properties of one to four units. Read MorePublic Health Policy Paper1548 Words   |  7 Pages Abstract This public health policy paper will discuss and outline the Affordable Care Act (ACA) as well as barriers and controversies surrounding the policy and its relevance in nursing profession. The ACA will eventually affect everyone. Statistics reflecting United States health outcomes have proven the need for the initiation of policy formation within the United States healthcare system. â€Å"In March 2010, President Obama signed into law a comprehensive health reform, theRead MoreThe Role Of Leichhardt Local Government Area1868 Words   |  8 Pagesdiverse background, they range from Australian, United Kingdom, New Zealand, Italian, United States of America, Ireland, Greece, Germany, China and South Africa. According to the council’s own database, majority of the populous is born in Australia and other English speaking countries. It is also known for its Italian heritage for which 6.4% of the residents claim they are of Italian ancestry although the number has declined slightly over the last 10 years. 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A Outsider Of The Stranger By Andre Gide And Albert Camus

Andre Gide and Albert Camus seemingly had much in common. Both were French-speaking Nobel Prize winning writers with deep ties to France’s African territories and strong anti-establishment tendencies who sought to cast off the burdens society foisted upon them. Yet their as best evidenced by their respective best-known texts, Gide’s L Immoraliste (The Immoralist) and Camus’ L’Étranger (The Stranger). While both novels center around aloof young men hostile to the norms that society foists upon them, their stories are far from similar. Michel, the protagonist of The Immoralist, is a well educated, thrill-seeking gentleman who casts away his marriage and his fortunes to satiate his desires. Meursault, the eponymous outsider of The Stranger,†¦show more content†¦He travels around France and North Africa looking for a location that will light the spark in his life, yet fails to do so. While Michel’s pederasty and spousal neglect is hardly a dmirable, those actions are not what make him an immoralist. Making the conscious decision to forsake the values of others in favor of a personal code of conduct - in this case discarding notions of financial solvency and peer respect in favor of the pursuit of temporary moments of pleasure - is the ideal of the Nietzschean ubermensch, or at least Gide’s version of it. In contrast, the reader is given little to no information about Meursault’s journey into moral apathy and uncaringness. When the novel begins, he is already the hollow automaton single-mindedly devoted to simple amusements and base needs that he will remain until moments before his death. Meursault feels no need to adhere to the framework of behavior society has laid out for him, nor does he have the urge to create any sort of rationale or calling of his own. He is content to simply show up to his job to make a living wage, then spend his free time enjoying the simple pleasures of sun, surf, and sex. The scene in the novel that arguably shows Meursault at his happiest is his mid-day swim with his lover Marie. Mersault lavishes an entire page of details on the Marie’s body, â€Å"wanting her so badly† when he sees her in herShow MoreRelatedExistentialism vs Essentialism23287 Words   |  94 Pagesamp; Phenomenology * Existentialist Philosophers * ------------------------------------------------- Absurdism * The idea of the  absurd  is a common theme in many existentialist works, particularly in  Camus. Absurdity is the notion of contrast between two things. As Camus explains it in  The Myth of Sisyphus: * The absurd is born out of this confrontation between the human need and the unreasonable silence of the world. * This view, which is shared by  Sartre, is that humanity

Cell differentiation by mechanical stress

Questions: 1. Compare and contrast primary cells and cell lines. In your answer, make sure to address the following points? 2. Describe how cell lines are maintained in the lab. In your answer, make sure to address the following points? 3. Describe the process of differentiation. In your answer make sure to address the following points? Answers: 1. a. The preparation of primary cells for cell culture. i. The organ is isolated and selected (Stacey, Doyle, Alan, Ferro, Margherita, 2002).ii. The organ is dissected to remove fat and necrotic cellsiii. The cells are subjected to enzymatic degradation (Stacey et al., 2002).iv. Trypsin or collagenase is addedv. Re-suspend and seed the medium Conditions needed for cell to survive include; gas mixture and temperature of 5% CO2 and 37 degrees Celsius respectively, appropriate PH, glucose and nutrients (Stacey et al., 2002). Advantages of primary cells over in vivo i. Use of primary cell is cost effective ("Primary Cell Culture | Sigma-Aldrich," n.d.). ii. Primary cells use avoids ethical objections. Disadvantages of primary cells i. Primary cells take longer to grow ii. Culture and isolations costs for primary cells are prohibitive and high c. Cell lines originate from a cell culture made from a single cell. The main distinguishing features of cell lines from primary cells include, cell lines have minimal variability while primary cells have variability existing between preparations and the donor cells ("Primary Cell Culture | Sigma-Aldrich," n.d.). d. Advantages of using cell lines over primary cells in experimentation i. Cell lines take less time to grow unlike primary cells ii. Cell lines have minimal variability unlike in primary cells. Disadvantages of using cell line over primary cells in experimentations i. They provide less relevant results as compared to primary cells ("Primary Cell Culture | Sigma-Aldrich," n.d.). 2. i. Confluence is the approximate number of the adherent cells in the flask or dish of the cell culture (Mather, 2008). ii. The procedure of cell line passaging starts with the removal of the medium of the HEK 293 cell flask completely (Masters, Stacey, 2007). This is then followed by addition of 3ml trypsin to the medium. The next step is placing the flask containing the medium to an incubator at a temperature of 37 degrees Celsius for 5 minutes (Masters, Stacey, 2007). Then rock the flask and wash down the cells by addition of medium and pipette to separate cell clusters into single cell lines. Add desirable volume of re-suspended cell mix into new plates. Finally, shake the plates or the flask in gentle manner to allow proper mixing then place them back to CO2 incubator at temperature of 37 degrees Celsius (Masters, Stacey, 2007).Passing cell lines helps to evade senescence. Passaging a cell line too many times results in cells having little in common with original reference strain. Preparation of cell lines for cryopreservation i. Harvesting cells in the log growth phase (Stacey, Hawkins, Fleck, 2011).ii. Bring both the semi-adherent and adherent cells into suspension by the use of EDTAiii. Remove a small aliquot of the cells and do cell quantification iv. Perform centrifugation on the remaining culture for approximately 5 minutesv. Re-suspend the cells in the freeze medium vi. Then pipette one aliquot of the cells vii. Place the ampoules in a freezer whose rate is controlled viii. The medium is then shifted to a freezer for cryopreservation 3. i. Differentiation is a process that happens in multicellular organisms where a cell with less specialization matures so that it becomes more discrete in its functions and form (Altman, 2001). The major importance of differentiation in multicellular organisms is that it provides them with a variety of cell specialized in performing specific functions. ii. Somatic cells differentiation starts with the chromatin for both plants and animals. Chromatin reorganization is the first steps that occurs which in turn lead to activation of the pluripotent genes including Oct3/4 in animals and NAM in plants, establishing pluripotentiality (Nakagawa, M., Yamanaka, 2010). There are a variety of activities that lead to chromatin modelling induced pluripotentiality that may include activity of the thrombin in serum, cell wall removal and viral infections. Basically, the presence of these stimuli signals the cells to differentiate thus that are how the cells know when to differentiate. iii. Stem cells have the following characteristics: Ability to divide and renew themselves Basic and unspecialized cells (Miki et al., 2005). Give rise to specialized cells through differentiation (Mather, 2008). According to Baker (2008) stem cells are appealing to the medical community because of their therapeutic potential. References Altman,G.H. (2001). Cell differentiation by mechanical stress. The FASEB Journal. doi:10.1096/fj.01-0656fje Baker,M. (2008). Cancer and stem cells: Beckman conference. Nature Reports Stem Cells. doi:10.1038/stemcells.2008.47 Masters,J.R., Stacey,G.N. (2007). Changing medium and passaging cell lines. Nature Protocols, 2(9), 2276-2284. doi:10.1038/nprot.2007.319 Mather,J.P. (2008). Stem cell culture. London: Elsevier/Academic Press. Miki,T., Lehmann,T., Cai,H., Stolz,D.B., Strom,S.C. (2005). Stem Cell Characteristics of Amniotic Epithelial Cells. Stem Cells, 23(10), 1549-1559. doi:10.1634/stemcells.2004-0357 Nakagawa,M., Yamanaka,S. (2010). Reprogramming of Somatic Cells to Pluripotency. Advances in Experimental Medicine and Biology, 215-224. doi:10.1007/978-1-4419-7037-4_14 Primary Cell Culture | Sigma-Aldrich. (n.d.). Retrieved from https://www.sigmaaldrich.com/technical-documents/articles/biology/primary-cell-culture.html Stacey,G., Doyle, Alan, Ferro, Margherita. (2002). Cell Culture Methods for in Vitro Toxicology. Springer Verlag. Stacey,G.N., Hawkins,R., Fleck,R.A. (2011). Cryopreservation and Banking of Cell Lines. Animal Cell Culture, 185-203. doi:10.1002/9780470669815.ch6

Australian Fashion Design and Cultural Identity- MyAssignmenthelp

Question: Discuss about theAustralian Fashion Design and Cultural Identity. Answer: Introduction Fashion is shaped on the basis of national culture. However, based on the historical foundations, the Australian fashion is mainly shaped through the models from Britain, which have been evolved internationally. However, the modern Australia has a unique style of fashion, which is able to be clearly distinguished from European fashion trends. It has been revealed from the fashion brands principles in Australia; the designers are continuously trying to keep balance between creativity and national identity (Entwistle, 2015). All the designer brands attempt to integrate unique features, symbolizing the particular cultural identities. In Australian culture, fashion is distinguished from dress not by only how the fashion been fashioned or created rather by its nature. Moreover, fashion is represented by reflecting the prevailing styles in polite society instead or being based on function. Identity is one of the most compelling and contentious concepts in humanities and social science. Fas hion is significantly linked with constructing and reconstructing identity, i.e. how people represent the contradictions and self in their daily life. Through appearance and style people represents their wishes and thus it is a metaphor for identity (Bulmer Buchanan-Oliver, 2010). While considering a particular fashion cloth, i.e. skirt, Australian culture significantly represents a difference from the European culture. For instance, Australian culture believes in wearing such cloths that would help them to be relaxed. Thus, in contrast to the full or knee length skirts preferred by the European culture, the cloth has evolved to miniskirts in Australian culture. In also the indigenous culture, skirts are favoured in Australia, although the materials for those clothes are different from the non-indigenous Australian culture. Moreover, Australian culture promotes colourful representation and establishing a link with nature (Gibson, 2016). Thus the floral and fabric work is preferred in skirts. The fashion appearance in Australia has been represented the influence from 1960s fashion design and those clothes are modified and integrated in new forms in the current Australian fashion culture. For instance, during 1960s, the sarong design became very popular among th e Australian people. Additionally, during 1975, specialized hand prints in the t-shirts, sarongs and accessories have been shown to be prevalent. According to the trends and tradition of sarong skirts currently Australian labels are exploring their designs to boutiques and department stores in Europe, Asia, US and other countries throughout the world (Geczy Karaminas, 2012). Therefore, Australian brands are attempting to promote their global presence, which included Gorman brand for their unique style of skirt. Fashion weeks at Sydney and Melbourne represent different unique combination of Australian tradition with the modern clothing principles like balance between colour, texture, cutting, pattern and combination. In this assignment of Australian clothing and fashion culture analysis, the renowned fashion clothing brand has been selected, i.e. the Gorman brand, which have significantly looking for aligning their modern fashion and clothing designs with the Australian culture and evolving lifestyle. In this context, the brand especially focuses upon maintaining the quality of fabrics, shapes and customized prints, which modern Australian generation is looking for, while maintaining a link with the national culture (Healey, 2010). The founder of the brand, i.e. Lisa Gorman launcher her eponymous label at fledgling boutique in 1999 in Melbourne, with her latest collection known as less than 12 degrees, which got an instant success. The entrepreneur identified something special in her brand and followed that unique trend in her designs through years; as a result Gorman label became an iconic part of the Australian fashion industry. The reason behind its flourish is the unique thought of the owner to make a strong link between the traditional Australian culture and the specialized needs of modern young generation through the unique texture, fibre and colour concepts. Although the organization was started at Melbourne, which is limited within the home country, the online presence of the brand is making its designer clothes available to women throughout the world. Starting from a status as one-lady-with-sewing-machine-in-her-bedroom the founder has adopted unique way to turn her small business into a major operation, without slowing down (Wilcox, 2017). The brand, with other womens outfit, prioritizes skirts, which is a preferred outfit by the Australian women as casual, professional as well as occasional outfit. It is in the fashion since 1960s and the brand Garmon is attempting to keep an essence of Australian traditional symbol in the modern skirt design. For instance, The philosophy of the organization is that it consist a distinct handwriting underpinning all of the designs, including its signature prints, directional knitwear and distinctive colour palettes (Gorman Online, 2017). In addition, the brand is thoroughly modern for all of its tribal and vintage references and the inspiration from the local culture and artist surrounding the brand is significantly inspiring its designs, especially the culture of the founders local fitzroy. In addition, to symbolize the culture in the clothing, the flora and fauna, which are native to the continent, like Kangaroo, emu, wattle are used sometimes in designs. For instance, in sarongs, these symbols are commonly utilized in designs. The brand uses different types of fabrics and textures, aligning with the style and pattern suitable for the residents. The founder inspires the designers to include their cultural experience in their designs. For instance, the city, the person or a song along with a touch of m odernism. In addition, Australians are nature lover and like to be relaxed, thus the brand attempts to incorporate natural art, i.e. sunset or flower, representing each designs with colourful background, as per the likings of people (McRobbie, 2013). The organization includes silhouette prints for representing relaxed mood, floral and other colourful patters for playful representation along with a urban touch in their materials and colours. The unique pattern of the skirts also includes a beachy and natural feel. The formal collection for skirts is made on the basis of visual communication principles, like maintaining a proper balance between colours. As equal distribution of colour makes communication boring, unequal distribution of colours would be required (Garling et al., 2013). The patterns are unique for the brand that is why the brand is getting global appearance quickly. On the other hand, the casual skirts, like wrap over, miniskirts and sarong and knee length skirts include feature rope, coral, rock patterns, slit, star fish or shells and loose fabric. To represent more on culture and climate, they also launches a seasonal clothes, for instance, in summer or spring season, light pattern and fabric is used for creating more refreshing and colourful designs, according to their relaxing nature (Carrico Kim, 2014). For this, they use light soft fabric with lace and overlays, which gives an essence of feminine loo k. On the other hand, the formal skirts are more sensitively designed to eliminate any symbols of boldness. In addition to the consideration of cultural attributes, the organization is also focusing upon the social well being also. For instance, the brand introduced Gorman organic in 2007, for responding towards increasing environmental awareness. Thus, the organization started to focus more on manufacturing innovative organic fabric. In addition, the organization blended their innovation throughout their traditional cultural collection, thereby offering customers to make a sustainable choice, instead of compromising good design and quality. In this context, all the garments produced and offered by the brand are made of certified organic materials or comes from sustainable farming or non-chemical processing of via closed loop production means. In this context, to be culturally identical, the designs and patterns are made unique, which can be significantly distinguishable from the other nations Yu et al., 2014). However, the brand follows the traditional western culture through their global presence. The visual communication is aligned with the cultural traits, aligning with the design principle and values. For instance, the traditional occasional skirts are made through the use of warm and dark colours, as these colours contain more visual weight than cool and light colours. The combination of colour and patterns follows the 6 design principles and values. Longer, wider lines are included, while maintaining a visual balance in colour, texture, pattern and shape in formal and party wears, whereas for beach or summer skirts, the short and thinner lines along with soft, fine textures are used (Baird Collias, 2014). Figure: Gorman skirts (left: relaxed, middle: professional, right: occasional) (Source: Gorman Online, 2017) Analyzing the cultural attributes of Australian cloth company Gorman, it has been revealed that the brand has several strength as well as weaknesses. For instance, the organization is continuously working on improving their production, through innovation and making their products customer-centric as well as environment friendly, as a result the brand has a significant chance of global expansion. However, one of its key weaknesses is less offline presence; the brand is providing more concentration on its online presence. Thus, it can be interpreted that this area should be improved significantly. Reference List Baird, D. G., Collias, D. I. (2014).Polymer processing: principles and design. John Wiley Sons. Bulmer, S., Buchanan-Oliver, M. (2010). Experiences of brands and national identity. Australian Marketing Journal 18(4), 199-205. Carrico, M., Kim, V. (2014). Expanding zero-waste design practices: a discussion paper.International Journal of Fashion Design, Technology and Education,7(1), 58-64. Entwistle, J. (2015). The fashioned body: Fashion, dress, and social theory. John Wiley Sons. Fletcher, K. (2010). Slow fashion: An invitation for systems change. Fashion practice. The Journal of Design, Creative Process and the Fashion Industry, 259-266. Garling, S., Hunt, J., Smith, D., Sanders, W. (2013).Contested governance: culture, power and institutions in Indigenous Australia(p. 351). ANU Press. Geczy, A., Karaminas, V. (2012). Fashion and art. London: Berg. Gibson, C. (2016). How clothing design and cultural industries refashioned frontier masculinities: a historical geography of Western wear.Gender, Place Culture,23(5), 733-752. Gorman Online. (2017).Gorman Online :: About Us.Gorman Online. Retrieved 2 June 2017, from https://www.gormanshop.com.au/aboutus/ Gregory, D. (2004). It's all about Australia, mate. Auckland: Exisle Publishers. Healey, R. (2010). Global positioning of Australian fashion. New York: Berg. McRobbie, A. (2013).In the culture society: Art, fashion and popular music. Routledge. Wilcox, C. (2017). Badge, boot, button: The story of Australian uniforms. Canberra: National Library of Australia. Yu, W., Fan, J., Ng, S. P., Harlock, S. (2014).Innovation and technology of women's intimate apparel. Woodhead Publishing.